FCoVCHECK Ab ELISA
ELISA kit for the detection of anti-feline Coronavirus antibodies
This kit is based on the indirect ELISA immunoassay technique for the detection of anti-FCoV antibodies in feline serum or plasma.
Compared to the reference test, FCoVCHECK Ab agreed at 96.4%, with 93.5% sensitivity (95% confidence interval (CI): 83.5% – 97.9%) and 100% specificity (95% CI: 90.8% – 100%). This new test agreed at 93.6% with ImmunoComb FCoV and 82.7% with INgezim Corona Felino.
The new ELISA test FCoVCHECK Ab correctly identifies positive and negative samples with a good correlation with IFAT, making it a good diagnostic aid for FIP.
References:
- DEVELOPMENT OF A NEW ELISA TEST FOR THE DETECTION OF ANTIBODIES ANTI-FELINE CORONAVIRUS AS A DIAGNOSTIC SUPPORT FOR FELINE INFECTIOUS PERITONITIS (FIP)
Irene Ferrero(1), Sarah Dewilde(2), Paolo Poletti(1), Barbara Canepa(2), Enrica Vittoria Giachino(1), Paola Dall'Ara(3), Joel Filipe(3)
(1) Agrolabo S.p.A, Research And Development, Scarmagno (TO), Italy, (2) Gem Forlab SRL, R&d, Caluso, Italy, (3) Università degli Studi di Milano, Medicina Veterinaria E Scienze Animali, Lodi, Italy
48th World Small Animal Veterinary Association Congress and 28th FECAVA Eurocongress - Development of a New Indirect ELISA Test for the Detection of Anti-Feline Coronavirus Antibodies in Cats
Irene Ferrero(1), Sarah Dewilde(2), Paolo Poletti(1), Barbara Canepa(2), Enrica Giachino(1), Paola Dall'Ara(3), and Joel Filipe(3)
(1) Agrolabo S.p.A., 10010 Scarmagno, Italy
(2) Gem Forlab S.r.l., 10014 Caluso, Italy
(3) Department of Veterinary Medicine and Animal Sciences, University of Milan, 26900 Lodi, Italy
Veterinary Scinces, Vet. Sci. 2025, 12, 245. https://doi.org/10.3390/vetsci12030245
Feline Infectious Peritonitis (FIP) is a disease caused by feline coronavirus (FCoV). In most cases, FCoV does not cause significant problems: they live in the intestine and are transported in the body by normal blood monocytes. The transmission of virus from cat to cat occurs through faeces and fecal contamination of the environment, and is therefore more likely in environments where there are numerous animals (farms, colonies, etc…). During the replication in the intestine, FCoV can mutate and give rise to the variants, which can cause FIP. Serology (IC, ELISA, Immunofluorescence) or PCR (Polymerase Chain Reaction, a technique that highlights the genome of the virus) performed on the blood can only tell if a cat has FCoV, but not if the virus present is the intestinal or mutated one. On the contrary, in the full-blown stages of the disease cats can be seronegative because antibodies are "seized" in the lesions. On the other hand, antibody titers may be high even in the absence of disease in kittens that have received antibodies from their mother or in breeding cats where, due to continuous re-infections, the titers fluctuate over time.
All these characteristics make serology and PCR not always useful for diagnosis in sick cats, but essential to control the spread of the virus in the breeding and therefore, to prevent the onset of the disease.
