EhrlichiaCHECK Ab ELISA
ELISA kit for the detection of anti-Ehrlichia canis antibodies
This kit is based on the indirect ELISA immunoassay technique for the detection of anti-Ehrlichia canis antibodies in canine serum or plasma.
Compared to IFAT, EhrlichiaCHECK Ab agreed at 99.2%, with 93.9% sensitivity (95% confidence interval (CI): 82.1% – 98.4%) and 100% specificity (95% CI: 94.2% – 100%). This new test agreed with INgezim Ehrlichia at 96.2%. Intra- and inter-assay test accuracy gave coefficients of variation lower than 10%.
The new ELISA test EhrlichiaCHECK Ab correctly identifies positive and negative samples with an excellent correlation with IFAT in a quick and easy way, with both visual and spectrophotometric reading.
References:
- EVALUATION OF A NEW ELISA TEST FOR THE DETERMINATION OF ANTIBODIES AGAINST EHRLICHIA CANIS
Irene Ferrero(1), Paolo Poletti(1), Enrica Vittoria Giachino(1), Paola Dall'Ara(2), Joel Filipe(2)
(1) Agrolabo S.p.A, Research And Development, Scarmagno (TO), Italy, (2) Università degli Studi di Milano, Medicina Veterinaria E Scienze Animali, Lodi, Italy
48th World Small Animal Veterinary Association Congress and 28th FECAVA Eurocongress - Evaluation of a newly developed rapid ELISA to detect anti-Ehrlichia canis antibodies in dogs
Irene Ferrero(1), Paolo Poletti(1), Enrica Giachino(1), Paola Dall'Ara(2), and Joel Filipe(2)
(1) Agrolabo S.p.A., 10010 Scarmagno, Italy
(2) Department of Veterinary Medicine and Animal Sciences, University of Milan, 26900 Lodi, Italy
Parasite 32, 62 (2025), published by EDP Sciences, 2025 – https://doi.org/10.1051/parasite/2025054
Canine ehrlichiosis is a tick-borne disease caused by Ehrlichia canis, an intracellular parasite belonging to the Rickettsial genus, which infects red blood cells up to cause alteration of their functioning and destruction of platelets.
Ticks become infected by taking blood from a dog presenting with the disease, both in the larvae and nymph stages, act as a reservoir of disease and can transmit the infection to sensitive dogs for at least 150 days after acquiring the infection .
Erlichiosis has a worldwide spread; in Europe it is mainly present in Italy, Spain and Portugal.
The evolution and outcome of the infection depend on a large variety of factors, including the bacterial load received. The presence of concomitant diseases, sustained by other parasites carried by ticks or other pathogens, can influence the manifestations and severity.
There are no age or sex predispositions for ehrlichiosis, however the German Shepherd seems to be more susceptible than other breeds.
The course of the disease was divided into three phases: acute, subclinical and chronic, based on clinical symptoms and clinical-pathological abnormalities.
The acute phase has a duration of 2 – 4 weeks, during which it is possible to observe symptoms such as:
- fever,
- oculo-nasal discharge,
- anorexia
- depression, lethargy,
- petechiae,
- bruising,
- lymphoadenomegaly in 20% of cases,
- splenomegaly in 25% of cases.
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Most dogs recover from the acute form with appropriate therapy.
Untreated dogs can undergo the subclinical phase.
In this form, body weight normalizes and pyrexia resolves. Dogs appear clinically healthy, however their platelet counts may remain below reference values. Dogs can stay in this phase for years.
The chronic form is characterized by a reduction in hematopoiesis, which can lead to pancytopenia. The prognosis in this phase is poor, death occurs from secondary infections and / or haemorrhage.
Other symptoms that can arise are:
- eye symptoms: change in the color and appearance of the eye, blindness, retinal diseases, anterior uveitis,
- neuromuscular symptoms: meningitis and / or haemorrhagic state, neuropathies, convulsions, atrophic muscles,
- polyarthritis,
- concomitant or secondary infections.
